![]() ![]() How are they calculated?Ī scale bar if often calculated at the time of when the drawing or map is complete, and before it is plotted (printed) to a set size. The one KEY and fixed factor is that they must be straight!. Graphically however, there tends to be a few key and standard methods of representation as shown below, however equally there can be a high level of creativity in the way that the measuring segments and units are shown. ![]() If the drawing is of a different scale, it requires a different scale bar to match.įor this reason and in terms of the units used as measurement, there are many variations. Scale bars are bespoke to the scale of the drawing or map they are assigned to, and can only be transferred between one drawing to another if both drawings are of the same ratio (scale).įor example a scale bar showing an imperial 1:8” scale, can only be used to measure a 1:8” drawing. This is particularly useful when measuring via a digital screen or mobile device, and when a plotter is not available to print the required paper size. This is because the scale bar, no matter how big or small the drawing becomes, maintains its proportion to the drawings subject. Why are they important?Īside from making map and drawing reading easier, when a scale bar is present, the drawing or map doesn’t necessarily need to be printed to a specific size. The addition of a scale bar however means that the drawing can be measured using a traditional ruler and/or via any other well proportioned object such as a credit card or posit.Īs for maps, these come in a variety of shapes and sizes that due to the large areas being shown, rarely represent a traditional drawing scale, and a scale bar is therefore the only way to accurately measure distance. ![]() Particularly within the design, architectural, and engineering professions, in many instances drawings are printed to an exact size and can therefore be measured via a scale rule. 53 164003, 2020.A scale bar is a linear graphic that is divided into equal segments and used to measure distances on drawings and/or maps that are produced to a set scale, but not necessarily printed to one. (1) “High photon count rates improve the quality of super-resolution fluorescence fluctuation spectroscopy” F. The STED WHITE 86x Water lens with motCORR also provides adaptive correction, as explained above, and is ideal for gentle live cell applications and STED-FCS (1).The STED WHITE 93x Glycerol lens provides adaptive correction for temperature fluctuations, refraction index mismatch, and inhomogeneities when looking deep in the sample, thanks to the motCORR fully motorized correction collar technology.The STED WHITE 100x oil lens delivers the highest resolution and offers excellent performance for daily imaging of fixed samples.Our STED WHITE Class of objective lenses have been especially developed to guarantee optimal conditions for a variety of STED experiments: In order to ensure you obtain the best brightness and resolution in 2D and 3D, we provide you with the best optics for assuring optimal overlay of the excitation and STED PSF all over the spectrum and that quantification capabilities are not lost due to adaptive corrections. With STELLARIS STED microscopes, you can obtain the results you need in both 2D and 3D as resolution becomes tunable in x, y and z. Additionally, the digital capabilities and speed of the Power HyD detectors, with 1.5 ns full-system dead time, guarantee that you get excellent image quality in terms of signal-to-noise and background, while delivering at least 10x more photon collection/pixel dwell time compared to APDs.ĢD and 3D STED imaging with outstanding brightness and resolution Our spectral detection offers you up to 5 STED-capable Power HyD detectors, so you can extend the number of molecular players to be localized in time and space. ![]() You can perform colocalization studies of multiple species and resolve them with subdiffraction detail. You can use the best dyes in the red region of the spectrum, go for fluorescent proteins in the green, or exploit novel fluorogenic probes in the orange. STED and STELLARIS excel at multicolor applications. Multicolor fluorescence labeling has the power to reveal relationships among intracellular species, because you can image different components with molecular specificity. Extend the number of simultaneous events you can study at the nanoscale ![]()
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